4. Chapter 4 microbial cell disruption

4.1. Basic teaching content

4.1 Composition and structure of cell wall 4.2 common crushing methods 4.3 determination of crushing rate.

4.2. Focus

  1. Calculation model of centrifugal sedimentation, physical concept of separation factor and application

4.3. Difficulties

  1. Structure and working principle of homogenizer.
  2. Structure and working principle of ball mill

4.4. The basic requirements of this chapter

  1. Master the common cell crushing methods and the cell wall composition of gramnegative cells;
  2. Master the principles and common equipment of biological, chemical, mechanical, and other methods